AP37,OsERF3

2022-11-15 | Categories genes  | Tags drought  stem  salinity  transcription factor  ABA  temperature  ethylene  growth  defense  grain  yield  insect  jasmonate  grain yield  brown planthopper  defense response  vegetative  drought tolerance  salt  crown root development  root development  root  auxin  cytokinin  crown root elongation  root elongation  crown root initiation  root initiation 

• Information
  • Symbol: AP37,OsERF3
  • MSU: LOC_Os01g58420
  • RAPdb: Os01g0797600
• PSP score
  • LOC_Os01g58420.1: 0.2487
• PLAAC score
  • LOC_Os01g58420.1: 0
• pLDDT score
  • 62.8
• Protein Structure from AlphaFold and UniProt
  • 
• Publication
  • Identification and characterization of a novel water-deficit-suppressed gene OsARD encoding an aci-reductone-dioxygenase-like protein in rice, 2005, Gene.
  • EAR motif mutation of rice OsERF3 alters the regulation of ethylene biosynthesis and drought tolerance, 2013, Planta.
  • An EAR-motif-containing ERF transcription factor affects herbivore-induced signaling, defense and resistance in rice, 2011, The Plant Journal.
  • Transcriptional activation of OsDERF1 in OsERF3 and OsAP2-39 negatively modulates ethylene synthesis and drought tolerance in rice, 2011, PLoS One.
  • Expression characteristics of OS-ACS1 and OS-ACS2, two members of the 1-aminocyclopropane-1-carboxylate synthase gene family in rice Oryza sativa L. cv. Habiganj Aman II during partial submergence, 1997, Plant Mol Biol.
  • Isolation and identification of an AP2/ERF factor that binds an allelic cis-element of rice gene LRK6, 2011, Genet Res (Camb).
  • Overexpression of the Transcription Factor AP37 in Rice Improves Grain Yield under Drought Conditions, 2009, Plant Physiol.
• Genbank accession number
  • AK061380
  • AK069262
  • AK061380
  • AK069262
• Key message
  • The overexpression of AP37 and AP59 in rice under the control of the constitutive promoter OsCc1 increased the tolerance to drought and high salinity at the vegetative stage
  • More importantly, the OsCc1:AP37 plants showed significantly enhanced drought tolerance in the field, which increased grain yield by 16% to 57% over controls under severe drought conditions, yet exhibited no significant difference under normal growth conditions
  • Our results suggest that the AP37 gene has the potential to improve drought tolerance in rice without causing undesirable growth phenotypes
  • Overexpression of the Transcription Factor AP37 in Rice Improves Grain Yield under Drought Conditions
  • We discovered a nucleus-localized ERF gene in rice (Oryza sativa), OsERF3, that was rapidly up-regulated in response to feeding by the rice striped stem borer (SSB) Chilo suppressalis
  • These results suggest that OsERF3, an AP2 (APETALA 2 Gene)/ERF transcription factor, binds the LRK6 promoter at this new motif, which might cause differential expression of LRK6 in the 93-11/Nipponbare hybrid
  • Here, we first revealed that the expression of OsERF3 was induced by drought, salt, ACC and ABA treatment
  • Increased tolerance to low temperatures was observed only in OsCc1:AP37 plants
  • OsERF3 is a transcriptional repressor with an ethylene-responsive element-binding factor-associated amphiphilic repression (EAR) motif (F/LDLNxxP), which transcriptionally represses the ethylene emission and drought tolerance in rice
  • Thus, our data reveal that the EAR motif is required for OsERF3 to transcriptionally regulate the ethylene synthesis and drought tolerance in rice, providing new insight to the roles of ethylene-response factor proteins in regulating ethylene biosynthesis and stress response
  • EAR motif mutation of rice OsERF3 alters the regulation of ethylene biosynthesis and drought tolerance
  • We propose that OsERF3 affects early components of herbivore-induced defense responses by suppressing MAPK repressors and modulating JA, SA, ethylene and H(2)O(2) pathways as well as plant resistance
  • On the other hand, OsERF3 was slightly suppressed by the rice brown planthopper (BPH) Nilaparvata lugens (StÃ¥l) and increased susceptibility to this piercing sucking insect, possibly by suppressing H(2)O(2) biosynthesis
  • Our results also illustrate that OsERF3 acts as a central switch that gears the plant’s metabolism towards an appropriate response to chewing or piercing/sucking insects
  • Antisense and over-expression of OsERF3 revealed that it positively affects transcript levels of two mitogen-activated protein kinases (MAPKs) and two WRKY genes as well as concentrations of jasmonate (JA), salicylate (SA) and the activity of trypsin protease inhibitors (TrypPIs)
  • Moreover, overexpression of OsERF3/OsAP2-39 suppressed ethylene synthesis
  • In addition, application of ACC recovered the drought-sensitive phenotype in the lines overexpressing OsERF3, showing that ethylene production contributed to drought response in rice
  • Transcriptional activation of OsDERF1 in OsERF3 and OsAP2-39 negatively modulates ethylene synthesis and drought tolerance in rice
  • Functional analysis revealed that ERF3 was essential for crown root development and acts in auxin- and cytokinin-responsive gene expression.
  • Also, increased expression of ERF3 could partially complement wox11, indicating that the two genes functioned cooperatively to regulate crown root development.
  • Furthermore, ERF3-regulated RR2 expression was involved in crown root initiation, while the ERF3/WOX11 interaction likely repressed RR2 during crown root elongation.
• Connection
  • AP37~OsERF3, OsEIL1~MHZ6, Identification and characterization of a novel water-deficit-suppressed gene OsARD encoding an aci-reductone-dioxygenase-like protein in rice, Furthermore, the expression of two genes for ethylene signal transduction, ETR2 and EIN3, increased in these RNAi transgenic plants, whereas the expression of ERF3 was suppressed
  • AP37~OsERF3, ETR2~Os-ERL1~OsETR2, Identification and characterization of a novel water-deficit-suppressed gene OsARD encoding an aci-reductone-dioxygenase-like protein in rice, Furthermore, the expression of two genes for ethylene signal transduction, ETR2 and EIN3, increased in these RNAi transgenic plants, whereas the expression of ERF3 was suppressed
  • AP37~OsERF3, OsACS2, EAR motif mutation of rice OsERF3 alters the regulation of ethylene biosynthesis and drought tolerance, Specifically, ACS2 was up-regulated in mEAR lines compared with that in OE lines and WT plants, suggesting that the Leu/Ala substitution within the EAR motif resulted in loss of repression of OsERF3
  • AP37~OsERF3, OsAP2-39, Transcriptional activation of OsDERF1 in OsERF3 and OsAP2-39 negatively modulates ethylene synthesis and drought tolerance in rice, Biochemical and molecular approaches showed that OsDERF1 at least directly interacted with the GCC box in the promoters of ERF repressors OsERF3 and OsAP2-39
  • AP37~OsERF3, OsAP2-39, Transcriptional activation of OsDERF1 in OsERF3 and OsAP2-39 negatively modulates ethylene synthesis and drought tolerance in rice, Moreover, overexpression of OsERF3/OsAP2-39 suppressed ethylene synthesis
  • AP37~OsERF3, OsAP2-39, Transcriptional activation of OsDERF1 in OsERF3 and OsAP2-39 negatively modulates ethylene synthesis and drought tolerance in rice, Transcriptional activation of OsDERF1 in OsERF3 and OsAP2-39 negatively modulates ethylene synthesis and drought tolerance in rice
  • AP37~OsERF3, OsDERF1, Transcriptional activation of OsDERF1 in OsERF3 and OsAP2-39 negatively modulates ethylene synthesis and drought tolerance in rice, Biochemical and molecular approaches showed that OsDERF1 at least directly interacted with the GCC box in the promoters of ERF repressors OsERF3 and OsAP2-39
  • AP37~OsERF3, OsDERF1, Transcriptional activation of OsDERF1 in OsERF3 and OsAP2-39 negatively modulates ethylene synthesis and drought tolerance in rice, Transcriptional activation of OsDERF1 in OsERF3 and OsAP2-39 negatively modulates ethylene synthesis and drought tolerance in rice
  • AP37~OsERF3, AP59~OsBIERF3~OsAP59, Overexpression of the Transcription Factor AP37 in Rice Improves Grain Yield under Drought Conditions, Two genes, AP37 and AP59, representing subgroups I and II, respectively, were functionally characterized
  • AP37~OsERF3, AP59~OsBIERF3~OsAP59, Overexpression of the Transcription Factor AP37 in Rice Improves Grain Yield under Drought Conditions, The overexpression of AP37 and AP59 in rice under the control of the constitutive promoter OsCc1 increased the tolerance to drought and high salinity at the vegetative stage
  • AP37~OsERF3, AP59~OsBIERF3~OsAP59, Overexpression of the Transcription Factor AP37 in Rice Improves Grain Yield under Drought Conditions, Microarray experiments identified 10 and 38 genes that are up-regulated by AP37 and AP59, respectively, in addition to 37 genes that are commonly induced by both factors
  • AP37~OsERF3, TIP2~bHLH142, The bHLH142 Transcription Factor Coordinates with TDR1 to Modulate the Expression of EAT1 and Regulate Pollen Development in Rice, EAT1 positively regulates the expression of AP37 and AP25, which induce tapetal programmed cell death.
  • AP37~OsERF3, WOX11, The Interaction between Rice ERF3 and WOX11 Promotes Crown Root Development by Regulating Gene Expression Involved in Cytokinin Signaling, Here, we identified a rice AP2/ERF protein, ERF3, which interacts with WOX11 and was expressed in crown root initials and during crown root growth.
  • AP37~OsERF3, WOX11, The Interaction between Rice ERF3 and WOX11 Promotes Crown Root Development by Regulating Gene Expression Involved in Cytokinin Signaling, Also, increased expression of ERF3 could partially complement wox11, indicating that the two genes functioned cooperatively to regulate crown root development.
  • AP37~OsERF3, WOX11, The Interaction between Rice ERF3 and WOX11 Promotes Crown Root Development by Regulating Gene Expression Involved in Cytokinin Signaling, ERF3 and WOX11 shared a common target, the cytokinin-responsive gene RR2.
  • AP37~OsERF3, WOX11, The Interaction between Rice ERF3 and WOX11 Promotes Crown Root Development by Regulating Gene Expression Involved in Cytokinin Signaling, Furthermore, ERF3-regulated RR2 expression was involved in crown root initiation, while the ERF3/WOX11 interaction likely repressed RR2 during crown root elongation.
  • AP37~OsERF3, OsERF109, The ethylene response factor OsERF109 negatively affects ethylene biosynthesis and drought tolerance in rice., Quantitative real-time PCR analysis also proved the regulation of OsERF109 in the expression of OSACS6, OSACO2, and OsERF3, which have been identified to play important roles in ethylene biosynthesis

Prev     Next