BPH26,BPH18

2022-11-15 | Categories genes  | Tags resistance  map-based cloning  brown planthopper  phloem  leaf  vascular bundle  insect  R protein 

• Information
  • Symbol: BPH26,BPH18,BPH9
  • MSU: LOC_Os12g37280

  • RAPdb: Os12g0559400

  • MSU: LOC_Os12g37290
  • RAPdb: Os12g0559400
• PSP score
  • LOC_Os12g37280.1: 0.1216
  • LOC_Os12g37290.1: 0.006
• PLAAC score
  • LOC_Os12g37280.1: 0
  • LOC_Os12g37290.1: 0
• pLDDT score
  • 68.7
• Protein Structure from AlphaFold and UniProt
  • 
• Publication
  • Map-based cloning and characterization of a brown planthopper resistance gene BPH26 from Oryza sativa L. ssp. indica cultivar ADR52., 2014, Sci Rep.
  • Map-based Cloning and Characterization of the BPH18 Gene from Wild Rice Conferring Resistance to Brown Planthopper BPH Insect Pest., 2016, Sci Rep.
• Genbank accession number
  • AB910360
  • KF890252
  • KJ850252
  • AB910360
  • KF890252
  • KJ850252
  • AB910360
  • KF890252
  • KJ850252
• Key message
  • Map-based cloning and characterization of a brown planthopper resistance gene BPH26 from Oryza sativa L. ssp. indica cultivar ADR52.
  • The indica rice cultivar ADR52 carries two BPH resistance genes, BPH26 (brown planthopper resistance 26) and BPH25
  • Map-based cloning of BPH26 revealed that BPH26 encodes a coiled-coil-nucleotide-binding-site-leucine-rich repeat (CC-NBS-LRR) protein
  • BPH26 mediated sucking inhibition in the phloem sieve element
  • The BPH18 promoter::GUS transgenic plants exhibited strong GUS expression in the vascular bundles of the leaf sheath, especially in phloem cells where the BPH attacks
  • Map-based Cloning and Characterization of the BPH18 Gene from Wild Rice Conferring Resistance to Brown Planthopper (BPH) Insect Pest.
  • However, these two genes have remarkable sequence differences and the independent NILs showed differential BPH resistance with different expression patterns of plant defense-related genes, indicating that BPH18 and BPH26 are functionally different alleles
  • Map-based cloning and complementation test revealed that the BPH18 encodes CC-NBS-NBS-LRR protein
  • The BPH18 proteins were widely localized to the endo-membranes in a cell, including the endoplasmic reticulum, Golgi apparatus, trans-Golgi network, and prevacuolar compartments, suggesting that BPH18 may recognize the BPH invasion at endo-membranes in phloem cells
  • BPH18 has two NBS domains, unlike the typical NBS-LRR proteins
• Connection

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