CYP90D3

2022-11-15 | Categories genes  | Tags insect  brassinosteroid 

• Information
  • Symbol: CYP90D3
  • MSU: LOC_Os05g11130
  • RAPdb: Os05g0200400
• PSP score
  • LOC_Os05g11130.1: 0.0043
• PLAAC score
  • LOC_Os05g11130.1: 0
• pLDDT score
  • 90.03
• Protein Structure from AlphaFold and UniProt
  • 
• Publication
  • Rice CYP90D2 and CYP90D3 catalyze C-23 hydroxylation of brassinosteroids in vitro, 2012, Plant Physiol Biochem.
  • A rice brassinosteroid-deficient mutant, ebisu dwarf d2, is caused by a loss of function of a new member of cytochrome P450, 2003, Plant Cell.
• Genbank accession number
  • AAT44310
• Key message
  • However, in vitro biochemical assays using baculovirus/insect cell-produced proteins revealed that both CYP90D2 and CYP90D3 catalyze C-23 hydroxylation of various 22-hydroxylated brassinosteroids, but with markedly different catalytic efficiencies
  • We report the biochemical characterization of two brassinosteroid-biosynthetic P450s from rice: CYP90D2 and CYP90D3
  • These results indicate that CYP90D2 and CYP90D3 can act as brassinosteroid C-23 hydroxylases in rice
  • Rice CYP90D2 and CYP90D3 catalyze C-23 hydroxylation of brassinosteroids in vitro
• Connection
  • CYP90D3, D2~CYP90D2~SMG11, Rice CYP90D2 and CYP90D3 catalyze C-23 hydroxylation of brassinosteroids in vitro, We report the biochemical characterization of two brassinosteroid-biosynthetic P450s from rice: CYP90D2 and CYP90D3
  • CYP90D3, D2~CYP90D2~SMG11, Rice CYP90D2 and CYP90D3 catalyze C-23 hydroxylation of brassinosteroids in vitro, However, in vitro biochemical assays using baculovirus/insect cell-produced proteins revealed that both CYP90D2 and CYP90D3 catalyze C-23 hydroxylation of various 22-hydroxylated brassinosteroids, but with markedly different catalytic efficiencies
  • CYP90D3, D2~CYP90D2~SMG11, Rice CYP90D2 and CYP90D3 catalyze C-23 hydroxylation of brassinosteroids in vitro, These results indicate that CYP90D2 and CYP90D3 can act as brassinosteroid C-23 hydroxylases in rice
  • CYP90D3, D2~CYP90D2~SMG11, Rice CYP90D2 and CYP90D3 catalyze C-23 hydroxylation of brassinosteroids in vitro, Rice CYP90D2 and CYP90D3 catalyze C-23 hydroxylation of brassinosteroids in vitro

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