- Information
- Symbol: D35,OsKOS3,OsKO2
- MSU: LOC_Os06g37364
- RAPdb: Os06g0570100
- PSP score
- LOC_Os06g37364.2: 0.0065
- LOC_Os06g37364.3: 0.0037
- LOC_Os06g37364.1: 0.0026
- PLAAC score
- LOC_Os06g37364.2: 0
- LOC_Os06g37364.3: 0
- LOC_Os06g37364.1: 0
- pLDDT score
- 89.86
- Protein Structure from AlphaFold and UniProt
- Publication
- A rice semi-dwarf gene, Tan-Ginbozu D35, encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase, 2004, Plant Mol Biol.
- Functional Identification of a Riceent-Kaurene Oxidase, OsKO2, Using thePichia pastorisExpression System, 2014, Bioscience, Biotechnology and Biochemistry.
- Mutation of rice BC12/GDD1, which encodes a kinesin-like protein that binds to a GA biosynthesis gene promoter, leads to dwarfism with impaired cell elongation, 2011, Plant Cell.
- The rice dwarf virus P2 protein interacts with ent-kaurene oxidases in vivo, leading to reduced biosynthesis of gibberellins and rice dwarf symptoms, 2005, Plant Physiol.
- CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism, 2012, Plant Physiol.
- Overexpression of rice LRK1 restricts internode elongation by down-regulating OsKO2, 2013, Biotechnol Lett.
- Genbank accession number
- Key message
- ) semi-dwarf cultivar, Tan-Ginbozu (d35Tan-Ginbozu), contributed to the increase in crop productivity in Japan in the 1950s
- Previous studies suggested that the semi-dwarf stature of d35Tan-Ginbozu is caused by a defective early step of gibberellin biosynthesis, which is catalyzed by ent-kaurene oxidase (KO)
- Homozygote with null alleles of D35 showed a severe dwarf phenotype; therefore, d35Tan-Ginbozu is a weak allele of D35
- Introduction of OsKOL4 into d35Tan-Ginbozu did not rescue its dwarf phenotype, indicating that OsKOL4 is not involved in GA biosynthesis
- A rice semi-dwarf gene, Tan-Ginbozu (D35), encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase
- Comparing d35Tan-Ginbozu with other high yielding cultivars, we discuss strategies to produce culm architectures suitable for high crop yield by decreasing GA levels
- Rice (Oryza sativa) contains five CYP701A subfamily members in its genome, despite the fact that only one (OsKO2/CYP701A6) is required for GA biosynthesis
- In particular, under conditions where OsKO2 catalyzes the expected conversion of ent-kaurene to ent-kaurenoic acid required for GA biosynthesis, OsKOL4 instead efficiently reacts with ent-sandaracopimaradiene and ent-cassadiene to produce the corresponding C3alpha-hydroxylated diterpenoids
- Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis
- This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice
- Connection
- D35~OsKOS3~OsKO2, OsKOS1~OsKOL4~CYP701A8, CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism, In particular, under conditions where OsKO2 catalyzes the expected conversion of ent-kaurene to ent-kaurenoic acid required for GA biosynthesis, OsKOL4 instead efficiently reacts with ent-sandaracopimaradiene and ent-cassadiene to produce the corresponding C3alpha-hydroxylated diterpenoids
- D35~OsKOS3~OsKO2, OsNAC2~OsTIL1~OMTN2~OsORE1, OsNAC2 encoding a NAC transcription factor affects plant height through mediating the gibberellic acid pathway in rice., Dual-luciferase reporter analyses, yeast one-hybrid assays and ChIP-qPCR suggested that OsNAC2 directly interacted with the promoter of OsEATB and OsKO2
- D35~OsKOS3~OsKO2, OsbZIP48, OsbZIP48, a HY5 transcription factor ortholog, exerts pleiotropic effects in light-regulated development., Electrophoretic mobility shift assay showed that OsbZIP48 directly binds to the OsKO2 promoter
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