FLO7

• Symbol: FLO7
• MSU: LOC_Os10g32680
• RAPdb: Os10g0463800

• 88.82

• FLOURY ENDOSPERM7 encodes a regulator of starch synthesis and amyloplast development essential for peripheral endosperm development in rice., 2015, J Exp Bot.

• LOC_Os10g32680.3: 0.1031
• LOC_Os10g32680.2: 0.0293
• LOC_Os10g32680.1: 0.02

• LOC_Os10g32680.3: 0
• LOC_Os10g32680.2: 0
• LOC_Os10g32680.1: 0

Genbank accession number

• Notably, flo7 peripheral endosperm cells showed obvious defects in compound starch grain development
• Together, our findings identify FLO7 as a unique plant regulator required for starch synthesis and amyloplast development within the peripheral endosperm and provide new insights into the spatial regulation of endosperm development in rice
• Map-based cloning of FLO7 revealed that it encodes a protein of unknown function
• Consistent with the phenotypic alternation in flo7 endosperm, the flo7 mutant had reduced amylose content and seriously disrupted amylopectin structure only in the peripheral endosperm
• FLO7 harbors an N-terminal transit peptide capable of targeting functional FLO7 fused to green fluorescent protein to amyloplast stroma in developing endosperm cells, and a domain of unknown function 1338 (DUF1338) that is highly conserved in green plants
• Furthermore, our combined β-glucuronidase activity and RNA in situ hybridization assays showed that the FLO7 gene was expressed ubiquitously but exhibited a specific expression in the endosperm periphery
• Moreover, a set of in vivo experiments demonstrated that the missing 32 aa in the flo7 mutant protein are essential for the stable accumulation of FLO7 in the endosperm