- Information
- Symbol: ISA3
- MSU: LOC_Os09g29404
- RAPdb: Os09g0469400
- PSP score
- LOC_Os09g29404.3: 0.0197
- LOC_Os09g29404.2: 0.0227
- LOC_Os09g29404.1: 0.0197
- PLAAC score
- LOC_Os09g29404.3: 0
- LOC_Os09g29404.2: 0
- LOC_Os09g29404.1: 0
- pLDDT score
- 86.89
- Protein Structure from AlphaFold and UniProt
- Publication
- Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis, 2011, Plant Cell Physiol.
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Genbank accession number
- Key message
- Furthermore, chloroplasts in the leaf blade of isa3 seedlings were large and pleomorphic
- We investigated whether ISA3 plays a role in amyloplast development and starch metabolism in the developing endosperm
- ISA3-green fluorescent protein (GFP) fusion protein expressed under the control of the rice ISA3 promoter was targeted to the amyloplast stroma in the endosperm
- Both overexpression and loss of function of ISA3 in the endosperm generated pleomorphic amyloplasts and starch granules
- A transposon-inserted rice mutant of isoamylase3 (isa3) contained an increased amount of starch in the leaf blade at the end of the night, indicating that ISA3 plays a role in the degradation of transitory starch during the night
- Overexpression of ISA3 in the sugary1 mutant, which is deficient in ISA1 activity, did not convert water-soluble phytoglycogen to starch granules, indicating that ISA1 and ISA3 are not functionally redundant
- These results suggest that ISA3 facilitates starch metabolism and affects morphological characteristics of plastids in rice
- Connection
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