- Information
- Symbol: Lsi1,OsNIP2;1,OsLsi1
- MSU: LOC_Os02g51110
- RAPdb: Os02g0745100
- PSP score
- LOC_Os02g51110.1: 0.0208
- PLAAC score
- LOC_Os02g51110.1: 0
- pLDDT score
- 83.11
- Protein Structure from AlphaFold and UniProt
- Publication
- The role of the rice aquaporin Lsi1 in arsenite efflux from roots, 2010, New Phytol.
- A silicon transporter in rice, 2006, Nature.
- An efflux transporter of silicon in rice, 2007, Nature.
- Genotypic difference in silicon uptake and expression of silicon transporter genes in rice, 2007, Plant Physiol.
- Spatial distribution and temporal variation of the rice silicon transporter Lsi1, 2007, Plant Physiol.
- The rice aquaporin Lsi1 mediates uptake of methylated arsenic species, 2009, Plant Physiol.
- Involvement of silicon influx transporter OsNIP2;1 in selenite uptake in rice, 2010, Plant Physiol.
- Transporters of arsenite in rice and their role in arsenic accumulation in rice grain, 2008, Proc Natl Acad Sci U S A.
- Reduced arsenic accumulation in rice Oryza sativa L. shoot involves sulfur mediated improved thiol metabolism, antioxidant system and altered arsenic transporters., 2015, Plant Physiol Biochem.
- High silicon accumulation in the shoot is required for down-regulating the expression of Si transporter genes in rice., 2016, Plant Cell Physiol.
- High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice., 2016, Plant Cell Physiol.
- Overexpression of Lsi1 in cold-sensitive rice mediates transcriptional regulatory networks and enhances resistance to chilling stress, 2017, Plant Science.
- Genbank accession number
- Key message
- Mutation in OsNIP2;1 (Lsi1, a silicon influx transporter) significantly decreases arsenite uptake
- The rice (Oryza sativa) silicon transporter Lsi1 (OsNIP2;1, an aquaporin channel) is the major entry route of arsenite into rice roots
- Recently, a gene (Low silicon rice1 [Lsi1]) encoding a Si transporter was identified in rice roots
- Consistent with the Si uptake pattern, Lsi1 expression and distribution of the Lsi1 protein were found only in the basal zone of roots
- In the basal zones of the seminal, crown, and lateral roots, the Lsi1 protein showed a polar localization at the distal side of both the exodermis and endodermis, where the Casparian bands are formed
- Recent studies have shown that arsenite is taken up by rice (Oryza sativa) roots through two silicon transporters, Lsi1 (the aquaporin NIP2;1) and Lsi2 (an efflux carrier)
- The results demonstrate that Lsi1 mediates the uptake of undissociated methylated As in rice roots
- Herein, we show evidence that the uptake of selenite, a main bioavailable form of Se in paddy soils, is mediated by a silicon (Si) influx transporter Lsi1 (OsNIP2;1) in rice
- Immunostaining with Lsi1 and Lsi2 antibodies revealed a similar pattern of subcellular localization of these two Si transporters in both varieties; Lsi1 and Lsi2 were localized at the distal and proximal sides, respectively, of both exodermis and endodermis of the roots
- Whether Lsi1 also mediates arsenite efflux was investigated
- *Expression of Lsi1 in Xenopus laevis oocytes enhanced arsenite efflux, indicating that Lsi1 facilitates arsenite transport bidirectionally
- A rice mutant defective in Lsi1 (lsi1) extruded significantly less arsenite than the wild-type rice and, as a result, accumulated more arsenite in the roots
- *We conclude that Lsi1 plays a role in arsenite efflux in rice roots exposed to arsenate
- The role of the rice aquaporin Lsi1 in arsenite efflux from roots
- A gene, Lsi1, that encodes a silicon influx transporter has been identified in rice
- Here we describe a previously uncharacterized gene, low silicon rice 2 (Lsi2), which has no similarity to Lsi1
- Involvement of silicon influx transporter OsNIP2;1 in selenite uptake in rice
- Mutation in Lsi2 had a much greater impact on arsenic accumulation in shoots and grain in field-grown rice than Lsi1
- Spatial distribution and temporal variation of the rice silicon transporter Lsi1
- Taken together, our results indicate that Si influx transporter OsNIP2;1 is permeable to selenite
- The expression of two Si transporter genes (Low silicon rice 1 [Lsi1] and Lsi2) investigated using real-time reverse transcription polymerase chain reaction revealed higher expression of both genes in Nipponbare than in Kasalath
- Here we describe the Low silicon rice 1 (Lsi1) gene, which controls silicon accumulation in rice, a typical silicon-accumulating plant
- Suppression of Lsi1 expression resulted in reduced silicon uptake
- Furthermore, expression of Lsi1 in Xenopus oocytes showed transport activity for silicon only
- Defect of OsNIP2;1 resulted in a significant decrease in the Se concentration of the shoots and xylem sap when selenite was given
- There was a negative correlation between the expression level of OsLsi1 and OsLsi2 and shoot Si accumulation when the rice seedlings were exposed to different Si supply conditions
- A split root experiment showed that the expression of both OsLsi1 and OsLsi2 was also down-regulated in half the roots without direct Si exposure when the other half roots were exposed to Si
- In conclusion, the Si-induced down-regulation of Si transporter genes is controlled by shoot Si, not root Si, and that the region between -327 to -292 in the OsLsi1 promoter is involved in this regulation of OsLsi1 expression in rice
- A split root experiment showed that the expression of both OsLsi1 and OsLsi2 was also down-regulated in half the roots without direct Si exposure when the other half of the roots were exposed to Si
- In conclusion, the Si-induced down-regulation of Si transporter genes is controlled by shoot Si, not root Si, and the region between -327 and -292 in the OsLsi1 promoter is involved in this regulation of OsLsi1 expression in rice
- Our results suggest that when overexpressed Lsi1 in cold-sensitive rice, it possibility regulates the transcription factor OsWRKY53 in addition to the genes involved in the ROS metabolism, thus mediating resistance to chilling stress
- Overexpression of Lsi1 in cold-sensitive rice mediates transcriptional regulatory networks and enhances resistance to chilling stress
- Connection
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, An efflux transporter of silicon in rice, Here we describe a previously uncharacterized gene, low silicon rice 2 (Lsi2), which has no similarity to Lsi1
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, An efflux transporter of silicon in rice, The protein encoded by this gene is localized, like Lsi1, on the plasma membrane of cells in both the exodermis and the endodermis, but in contrast to Lsi1, which is localized on the distal side, Lsi2 is localized on the proximal side of the same cells
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Genotypic difference in silicon uptake and expression of silicon transporter genes in rice, The expression of two Si transporter genes (Low silicon rice 1 [Lsi1] and Lsi2) investigated using real-time reverse transcription polymerase chain reaction revealed higher expression of both genes in Nipponbare than in Kasalath
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Genotypic difference in silicon uptake and expression of silicon transporter genes in rice, Immunostaining with Lsi1 and Lsi2 antibodies revealed a similar pattern of subcellular localization of these two Si transporters in both varieties; Lsi1 and Lsi2 were localized at the distal and proximal sides, respectively, of both exodermis and endodermis of the roots
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, The rice aquaporin Lsi1 mediates uptake of methylated arsenic species, Recent studies have shown that arsenite is taken up by rice (Oryza sativa) roots through two silicon transporters, Lsi1 (the aquaporin NIP2;1) and Lsi2 (an efflux carrier)
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, The rice aquaporin Lsi1 mediates uptake of methylated arsenic species, The rice lsi1 mutant lost about 80% and 50% of the uptake capacity for MMA(V) and DMA(V), respectively, compared with the wild-type rice, whereas Lsi2 mutation had little effect
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Transporters of arsenite in rice and their role in arsenic accumulation in rice grain, Mutation in Lsi2 had a much greater impact on arsenic accumulation in shoots and grain in field-grown rice than Lsi1
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Reduced arsenic accumulation in rice Oryza sativa L. shoot involves sulfur mediated improved thiol metabolism, antioxidant system and altered arsenic transporters., High S treatment also reduced the expression of OsLsi1 and OsLsi2, the potent transporters of As in rice
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, High silicon accumulation in the shoot is required for down-regulating the expression of Si transporter genes in rice., The efficient uptake of Si in rice is mediated by two transporters OsLsi1 and OsLsi2, which function as influx and efflux transporters, respectively
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, High silicon accumulation in the shoot is required for down-regulating the expression of Si transporter genes in rice., Herein we investigated the mechanism underlying regulation of OsLsi1 and OsLsi2 expression
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, High silicon accumulation in the shoot is required for down-regulating the expression of Si transporter genes in rice., There was a negative correlation between the expression level of OsLsi1 and OsLsi2 and shoot Si accumulation when the rice seedlings were exposed to different Si supply conditions
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, High silicon accumulation in the shoot is required for down-regulating the expression of Si transporter genes in rice., A split root experiment showed that the expression of both OsLsi1 and OsLsi2 was also down-regulated in half the roots without direct Si exposure when the other half roots were exposed to Si
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice., The efficient uptake of Si in rice is mediated by two transporters OsLsi1 and OsLsi2, which function as influx and efflux transporters, respectively
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice., Herein we investigated the mechanism underlying regulation of OsLsi1 and OsLsi2 expression
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice., There was a negative correlation between the expression level of OsLsi1 and OsLsi2 and shoot Si accumulation when the rice seedlings were exposed to different Si supply conditions
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice., A split root experiment showed that the expression of both OsLsi1 and OsLsi2 was also down-regulated in half the roots without direct Si exposure when the other half of the roots were exposed to Si
- Lsi1~OsNIP2;1~OsLsi1, OsWRKY53, Overexpression of Lsi1 in cold-sensitive rice mediates transcriptional regulatory networks and enhances resistance to chilling stress, Our results suggest that when overexpressed Lsi1 in cold-sensitive rice, it possibility regulates the transcription factor OsWRKY53 in addition to the genes involved in the ROS metabolism, thus mediating resistance to chilling stress
Prev Next