OsAUX1

• Symbol: OsAUX1
• MSU: LOC_Os01g63770
• RAPdb: Os01g0856500

• LOC_Os01g63770.1: 0.045
• LOC_Os01g63770.2: 0.017

• LOC_Os01g63770.1: 0
• LOC_Os01g63770.2: 0

• 84.99

• OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice, 2012, PLoS One.
• Adenosine diphosphate ribosylation factor-GTPase-activating protein stimulates the transport of AUX1 endosome, which relies on actin cytoskeletal organization in rice root development, 2011, J Integr Plant Biol.
• OsAUX1 controls lateral root initiation in rice Oryza sativa L.., 2014, Plant Cell Environ.
• The auxin transporter, OsAUX1, is involved in primary root and root hair elongation and in Cd stress responses in rice Oryza sativa L.., 2015, Plant J.
• Dynamic Regulation of Auxin Response during Rice Development Revealed by Newly Established Hormone Biosensor Markers., 2017, Front Plant Sci.

Genbank accession number

• The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering
• OsAUX1 controls lateral root initiation in rice (Oryza sativa L.).
• The auxin influx carriers in Arabidopsis have been shown to control lateral root development and gravitropism, but little is known about these proteins in rice
• Three OsAUX1 T-DNA insertion mutants and RNAi knockdown transgenic plants reduced lateral root initiation compared to WT plants
• OsAUX1 overexpression plants exhibited increased lateral root initiation and OsAUX1 was highly expressed in lateral roots and lateral root primordia
• Similarly, the auxin reporter, DR5-GUS, was expressed at lower levels in osaux1 than in the WT plants, which indicated that the auxin levels in the mutant roots had decreased
• Overall, our results indicated that OsAUX1 was involved in polar auxin transport and functioned to control auxin-mediated lateral root initiation in rice
• Polar auxin transport, mediated by influx and efflux transporters, controls many aspects of plant growth and development
• Exogenous NAA treatment rescued the defective phenotype in osaux1-1 plants, whereas IAA and 2,4-D could not, which suggested that OsAUX1 was a putative auxin influx carrier
• The transcript levels of several auxin-signaling genes and cell cycle genes significantly declined in osaux1, hinting that the regulatory role of OsAUX1 may be mediated by auxin-signaling and cell cycle genes
• OsAUX1 expression in root hair cells is different from its paralogous gene, AtAUX1, expressed in non-hair cells
• Taken together, our results indicate that OsAUX1 plays an important role in root development and in responses to Cd stress
• However, OsAUX1 - like AtAUX1 - also localizes at the plasma membrane and seems to function as an auxin tranporter
• Decreased auxin distribution and contents in osaux1 mutant result in reduction of OsCyCB1;1 expression and shortened PR, LR and RH under Cd stress, but can be rescued by the membrane-permeable auxin, 1-naphthaleneacetic acid (NAA)
• Here, we characterize rice osaux1 mutants that have longer PR and shorter RH in hydroponic culture, and that are more sensitive to Cd stress compared to WT/Dongjin (DJ)
• Cd contents in the osaux1 mutant were not altered but reactive oxygen species (ROS)-mediated damage was enhanced, further increasing the osaux1 mutant sensitivity to Cd stress
• Moreover, protein localization of auxin transporters PIN1 homologs and GFP tagged OsAUX1 overlapped with DR5-VENUS during spikelet development, helping validate these auxin response reporters are reliable markers in rice

• OsAUX1, OsTB1~FC1~SCM3, OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice, The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering
• OsAUX1, OsTIR1, OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice, The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering
• OsAFB2, OsAUX1, OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice, The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering
• OsAGAP, OsAUX1, Adenosine diphosphate ribosylation factor-GTPase-activating protein stimulates the transport of AUX1 endosome, which relies on actin cytoskeletal organization in rice root development, Here, we report that overexpression of OsAGAP, an ARF-GTPase-activating protein in rice, stimulates vesicle transport from the plasma membrane to the Golgi apparatus in protoplasts and transgenic plants and induces the accumulation of early endosomes and AUX1
• OsAGAP, OsAUX1, Adenosine diphosphate ribosylation factor-GTPase-activating protein stimulates the transport of AUX1 endosome, which relies on actin cytoskeletal organization in rice root development, Fluorescence recovery after photobleaching analysis revealed exocytosis of the AUX1 recycling endosome was not affected in the OsAGAP overexpression cells, and was only slightly promoted when the actin filaments were completely disrupted by Lat B
• OsAGAP, OsAUX1, Adenosine diphosphate ribosylation factor-GTPase-activating protein stimulates the transport of AUX1 endosome, which relies on actin cytoskeletal organization in rice root development, Thus, we propose that AUX1 accumulation in the OsAGAP overexpression and actin disrupted cells may be due to the fact that endocytosis of the auxin influx carrier AUX1 early endosome was greatly promoted by actin cytoskeleton disruption