OsCERK1

2022-11-15 | Categories genes  | Tags defense response  defense  peptidoglycan and chitin signaling  chitin signaling  peptidoglycan signaling  innate immunity  chitin elicitor signaling pathway  immunity signalling  symbiosis  receptor kinase  immunity  blast  blast resistance  Kinase  root  calcium  epidermis 

• Information
  • Symbol: OsCERK1
  • MSU: LOC_Os08g42580
  • RAPdb: Os08g0538300
• PSP score
  • LOC_Os08g42580.1: 0.2026
  • LOC_Os08g42580.4: 0.0258
• PLAAC score
  • LOC_Os08g42580.1: 0
  • LOC_Os08g42580.4: 0
• pLDDT score
  • 82.12
• Protein Structure from AlphaFold and UniProt
  • 
• Publication
  • The Hop/Sti1-Hsp90 chaperone complex facilitates the maturation and transport of a PAMP receptor in rice innate immunity, 2010, Cell Host Microbe.
  • Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice, 2010, Plant J.
  • The receptor kinase CERK1 has dual functions in symbiosis and immunity signalling, 2014, Plant J.
  • The bifunctional plant receptor, OsCERK1, regulates both chitin-triggered immunity and arbuscular mycorrhizal symbiosis in rice., 2014, Plant Cell Physiol.
  • OsCERK1-mediated chitin perception and immune signaling requires Receptor-like Cytoplasmic Kinase 185 to activate a MAPK cascade in rice., 2017, Mol Plant.
  • The rice LysM receptor-like kinase OsCERK1 is required for the perception of short-chain chitin oligomers in arbuscular mycorrhizal signaling., 2017, New Phytol.
• Genbank accession number
  • AB510402
• Key message
  • Knockdown of OsCERK1 resulted in marked suppression of the defense responses induced by chitin oligosaccharides, indicating that OsCERK1 is essential for chitin signaling in rice
  • OsCERK1 and OsRLCK176 play important roles in peptidoglycan and chitin signaling in rice innate immunity
  • Here, we demonstrate that the rice LysM receptor-like kinase OsCERK1, a key component of the chitin elicitor signaling pathway, also plays an important role in PGN-triggered immunity in rice.
  • The receptor kinase CERK1 has dual functions in symbiosis and immunity signalling
  • CERK1 in rice has the highest homology to NFR1 and we show that this gene is also necessary for the establishment of the mycorrhizal interaction as well as for resistance to the rice blast fungus.
  • We show here that knockout (KO) mutants of OsCERK1, a rice receptor kinase essential for chitin signaling, were impaired not only for chitin-triggered defense responses but also for AM symbiosis, indicating the bifunctionality of OsCERK1 in defense and symbiosis
  • These results also suggest that OsCERK1 and homologs serve as a molecular switch that activates defense or symbiotic responses depending on the infecting microbes
  • On the other hand, a KO mutant of OsCEBiP, which forms a receptor complex with OsCERK1 and is essential for chitin-triggered immunity, established mycorrhizal symbiosis normally
  • Therefore, OsCERK1 but not chitin-triggered immunity is required for AM symbiosis
  • Furthermore, experiments with chimeric receptors showed that the kinase domains of OsCERK1 and homologs from non-leguminous, mycorrhizal plants could trigger nodulation signaling in legume-rhizobium interactions as the kinase domain of Nod factor receptor1 (NFR1), which is essential for triggering the nodulation program in leguminous plants, did
  • Here, we found that the PRR-associated receptor-like cytoplasmic kinase Oryza sativa RLCK185 transmits immune signaling from the PAMP receptor OsCERK1 to a MAPK signaling cascade through interaction with a MAPK kinase kinase, OsMAPKKK, which is the initial kinase of the MAPK cascade
  • Following the recent discovery that the Oscerk1 mutant is unable to host arbuscular mycorrhizal (AM) fungi, we have examined whether OsCERK1 is directly involved in the perception of the short-chain chitin oligomers (Myc-COs) identified in AM fungal exudates and shown to activate nuclear calcium (Ca(2+) ) spiking in the rice root epidermis
  • Compared with wild-type rice, Ca(2+) spiking responses to AM fungal elicitation were absent in root atrichoblasts of the Oscerk1 mutant
  • By contrast, rice lines mutated in OsCEBiP, encoding the LysM receptor-like protein which associates with OsCERK1 to perceive chitin elicitors of the host immune defense pathway, responded positively to Myc-COs
  • The rice LysM receptor-like kinase OsCERK1 is required for the perception of short-chain chitin oligomers in arbuscular mycorrhizal signaling.
  • The rice lysin-motif (LysM) receptor-like kinase OsCERK1 is now known to have a dual role in both pathogenic and symbiotic interactions
• Connection
  • Hsp90~rHsp90~OSGrp94, OsCERK1, The Hop/Sti1-Hsp90 chaperone complex facilitates the maturation and transport of a PAMP receptor in rice innate immunity, We find that the rice chitin receptor OsCERK1 interacts with Hsp90 and its cochaperone Hop/Sti1 in the endoplasmic reticulum (ER)
  • Hsp90~rHsp90~OSGrp94, OsCERK1, The Hop/Sti1-Hsp90 chaperone complex facilitates the maturation and transport of a PAMP receptor in rice innate immunity, Hop/Sti1 and Hsp90 are required for efficient transport of OsCERK1 from the ER to the plasma membrane (PM) via a pathway dependent on Sar1, a small GTPase which regulates ER-to-Golgi trafficking
  • CEBiP~OsCEBiP, OsCERK1, Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice, A plasma membrane glycoprotein, CEBiP (chitin elicitor binding protein) and a receptor kinase, CERK1 (chitin elicitor receptor kinase) (also known as LysM-RLK1), were identified as critical components for chitin signaling in rice and Arabidopsis, respectively
  • CEBiP~OsCEBiP, OsCERK1, Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice, We report here that rice cells require a LysM receptor-like kinase, OsCERK1, in addition to CEBiP, for chitin signaling
  • CEBiP~OsCEBiP, OsCERK1, Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice, The results of a yeast two-hybrid assay indicated that both CEBiP and OsCERK1 have the potential to form hetero- or homo-oligomers
  • CEBiP~OsCEBiP, OsCERK1, Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice, Immunoprecipitation using a membrane preparation from rice cells treated with chitin oligosaccharides suggested the ligand-induced formation of a receptor complex containing both CEBiP and OsCERK1
  • CEBiP~OsCEBiP, OsCERK1, Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice, Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice
  • LYP4~OsLYP4, OsCERK1, OsCERK1 and OsRLCK176 play important roles in peptidoglycan and chitin signaling in rice innate immunity, OsCERK1 associated with OsLYP4 or OsLYP6 when induced by PGN but it associated with OsLYP4, OsLYP6, or CEBiP under chitin treatment, suggesting the presence of different patterns of ligand-induced heterooligomeric receptor complexes.
  • LYP6~OsLYP6, OsCERK1, OsCERK1 and OsRLCK176 play important roles in peptidoglycan and chitin signaling in rice innate immunity, OsCERK1 associated with OsLYP4 or OsLYP6 when induced by PGN but it associated with OsLYP4, OsLYP6, or CEBiP under chitin treatment, suggesting the presence of different patterns of ligand-induced heterooligomeric receptor complexes.
  • CEBiP~OsCEBiP, OsCERK1, OsCERK1 and OsRLCK176 play important roles in peptidoglycan and chitin signaling in rice innate immunity, OsCERK1 associated with OsLYP4 or OsLYP6 when induced by PGN but it associated with OsLYP4, OsLYP6, or CEBiP under chitin treatment, suggesting the presence of different patterns of ligand-induced heterooligomeric receptor complexes.
  • OsCERK1, OsRLCK176, OsCERK1 and OsRLCK176 play important roles in peptidoglycan and chitin signaling in rice innate immunity, Furthermore, the receptor-like cytoplasmic kinase OsRLCK176 functions downstream of OsCERK1 in the PGN and chitin signaling pathways, suggesting that these MAMPs share overlapping intracellular signaling components.
  • CEBiP~OsCEBiP, OsCERK1, The bifunctional plant receptor, OsCERK1, regulates both chitin-triggered immunity and arbuscular mycorrhizal symbiosis in rice., On the other hand, a KO mutant of OsCEBiP, which forms a receptor complex with OsCERK1 and is essential for chitin-triggered immunity, established mycorrhizal symbiosis normally
  • OsCERK1, OsRacGEF1, An OsCEBiP/OsCERK1-OsRacGEF1-OsRac1 module is an essential early component of chitin-induced rice immunity., OsRacGEF1 interacts with OsCERK1 and is activated when its C-terminal S549 is phosphorylated by the cytoplasmic domain of OsCERK1 in response to chitin
  • OsCERK1, OsRacGEF1, An OsCEBiP/OsCERK1-OsRacGEF1-OsRac1 module is an essential early component of chitin-induced rice immunity., Further, a protein complex including OsCERK1 and OsRacGEF1 is transported from the endoplasmic reticulum to the PM
  • OsCERK1, OsRacGEF1, An OsCEBiP/OsCERK1-OsRacGEF1-OsRac1 module is an essential early component of chitin-induced rice immunity., Collectively, our results suggest that OsCEBiP, OsCERK1, OsRacGEF1, and OsRac1 function as key components of a "defensome" critically engaged early during chitin-induced immunity
  • OsCERK1, OsRacGEF1, New insights into the dimerization of small GTPase Rac/ROP guanine nucleotide exchange factors in rice., OsRacGEF2 does not bind directly to the receptor-like kinase (RLK) OsCERK1, but forms a complex with OsCERK1 through OsRacGEF1 at the ER
  • OsCERK1, OsRacGEF2, New insights into the dimerization of small GTPase Rac/ROP guanine nucleotide exchange factors in rice., OsRacGEF2 does not bind directly to the receptor-like kinase (RLK) OsCERK1, but forms a complex with OsCERK1 through OsRacGEF1 at the ER
  • OsCERK1, OsRacGEF1, Crosstalk of Signaling Mechanisms Involved in Host Defense and Symbiosis Against Microorganisms in Rice., In chitin signaling in rice, two direct substrates of OsCERK1, Rac/ROP GTPase guanine nucleotide exchange factor OsRacGEF1 and receptor-like cytoplasmic kinase OsRLCK185, have been identified as components of the OsCERK1 complex and are rapidly phosphorylated by OsCERK1 in response to chitin
  • OsCERK1, OsRLCK185, OsCERK1-mediated chitin perception and immune signaling requires Receptor-like Cytoplasmic Kinase 185 to activate a MAPK cascade in rice, OsCERK1-mediated chitin perception and immune signaling requires Receptor-like Cytoplasmic Kinase 185 to activate a MAPK cascade in rice
  • CEBiP~OsCEBiP, OsCERK1, The rice LysM receptor-like kinase OsCERK1 is required for the perception of short-chain chitin oligomers in arbuscular mycorrhizal signaling., By contrast, rice lines mutated in OsCEBiP, encoding the LysM receptor-like protein which associates with OsCERK1 to perceive chitin elicitors of the host immune defense pathway, responded positively to Myc-COs

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