- Information
- Symbol: OsCPK21
- MSU: LOC_Os08g42750
- RAPdb: Os08g0540400
- PSP score
- LOC_Os08g42750.1: 0.0067
- PLAAC score
- LOC_Os08g42750.1: 0
- pLDDT score
- 73.5
- Protein Structure from AlphaFold and UniProt
- Publication
- Functional characterisation of OsCPK21, a calcium-dependent protein kinase that confers salt tolerance in rice, 2011, Plant Mol Biol.
- Calcium-dependent protein kinase 21 phosphorylates 14-3-3 proteins in response to ABA signaling and salt stress in rice., 2017, Biochem Biophys Res Commun.
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Genbank accession number
- Key message
- These results suggest that OsCPK21 is involved in the positive regulation of the signaling pathways that are involved in the response to ABA and salt stress
- Functional characterisation of OsCPK21, a calcium-dependent protein kinase that confers salt tolerance in rice
- The survival rate of the OsCPK21-FOX plants was higher than that of wild-type (WT) plants grown under high salinity conditions
- Several ABA- and high salinity-inducible genes were more highly expressed in the OsCPK21-FOX plants than in WT plants
- The inhibition of seedling growth by abscisic acid (ABA) treatment was greater in the OsCPK21-FOX plants than in WT plants
- These results suggest that OsCPK21 phosphorylates OsGF14e to facilitate the response to ABA and salt stress
- We used an in<U+00A0>vitro kinase assay and site-directed mutagenesis to verify that OsCPK21 phosphorylates OsGF14e at Tyr-138
- Connection
- OsCPK21, OsGF14e~GF14e~GID2, Calcium-dependent protein kinase 21 phosphorylates 14-3-3 proteins in response to ABA signaling and salt stress in rice., In the present study, we performed yeast two-hybrid screening, glutathione S-transferase pull-down, co-immunoprecipitation, and bimolecular fluorescence complementation assays to confirm the interaction between OsCPK21 and one of its putative targets, Os14-3-3 (OsGF14e)
- OsCPK21, OsGF14e~GF14e~GID2, Calcium-dependent protein kinase 21 phosphorylates 14-3-3 proteins in response to ABA signaling and salt stress in rice., We used an in<U+00A0>vitro kinase assay and site-directed mutagenesis to verify that OsCPK21 phosphorylates OsGF14e at Tyr-138
- OsCPK21, OsGF14e~GF14e~GID2, Calcium-dependent protein kinase 21 phosphorylates 14-3-3 proteins in response to ABA signaling and salt stress in rice., These results suggest that OsCPK21 phosphorylates OsGF14e to facilitate the response to ABA and salt stress
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