OsCPS4,OsCyc1

2022-11-15 | Categories genes  | Tags growth  ga  gibberellin 

• Information
  • Symbol: OsCPS4,OsCyc1
  • MSU: LOC_Os04g09900
  • RAPdb: Os04g0178300
• PSP score
  • LOC_Os04g09900.1: 0.0618
  • LOC_Os04g09900.3: 0.006
  • LOC_Os04g09900.2: 0.0165
• PLAAC score
  • LOC_Os04g09900.1: 0
  • LOC_Os04g09900.3: 0
  • LOC_Os04g09900.2: 0
• pLDDT score
  • 85.75
• Protein Structure from AlphaFold and UniProt
  • 
• Publication
  • Cloning and Characterization of cDNAs Encodingent-Copalyl Diphosphate Synthases in Wheat: Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes, 2014, Bioscience, Biotechnology and Biochemistry.
  • OsTGAP1, a bZIP transcription factor, coordinately regulates the inductive production of diterpenoid phytoalexins in rice, 2009, J Biol Chem.
  • gid1, a gibberellin-insensitive dwarf mutant, shows altered regulation of probenazole-inducible protein PBZ1 in response to cold stress and pathogen attack, 2006, Plant, Cell and Environment.
  • Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis, 2004, Plant J.
  • Genetic evidence for natural product-mediated plant-plant allelopathy in rice Oryza sativa, 2012, New Phytol.
• Genbank accession number
  • AY530101
• Key message
  • Here, we apply reverse genetics, using knock-outs of the relevant diterpene synthases (copalyl diphosphate synthase 4 (OsCPS4) and kaurene synthase-like 4 (OsKSL4)), to demonstrate that rice momilactones are involved in allelopathy, including suppressing growth of the widespread rice paddy weed, barnyard grass (Echinochloa crus-galli)
  • Specifically, OsCPS1, which encodes an enzyme in the GA biosynthesis pathway, was down-regulated and OsCPS2 and OsCPS4, which encode enzymes in phytoalexin biosynthesis, were up-regulated in gid1
  • The phylogenetic tree and expression analyses suggest that TaCPS3 is responsible for gibberellin biosynthesis, while TaCPS1 and TaCPS2 are possible functional homologs of diterpene cyclase genes OsCPS2 and OsCPS4 involved in phytoalexin biosynthesis in rice
• Connection
  • OsCPS2~OsCyc2, OsCPS4~OsCyc1, Cloning and Characterization of cDNAs Encodingent-Copalyl Diphosphate Synthases in Wheat: Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes, The phylogenetic tree and expression analyses suggest that TaCPS3 is responsible for gibberellin biosynthesis, while TaCPS1 and TaCPS2 are possible functional homologs of diterpene cyclase genes OsCPS2 and OsCPS4 involved in phytoalexin biosynthesis in rice
  • OsCPS~OsCPS1, OsCPS4~OsCyc1, Cloning and Characterization of cDNAs Encodingent-Copalyl Diphosphate Synthases in Wheat: Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes, The phylogenetic tree and expression analyses suggest that TaCPS3 is responsible for gibberellin biosynthesis, while TaCPS1 and TaCPS2 are possible functional homologs of diterpene cyclase genes OsCPS2 and OsCPS4 involved in phytoalexin biosynthesis in rice
  • CYP99A3, OsCPS4~OsCyc1, OsTGAP1, a bZIP transcription factor, coordinately regulates the inductive production of diterpenoid phytoalexins in rice, The knock-out mutant for OsTGAP1 had almost no expression of the five clustered genes (OsCPS4, OsKSL4, CYP99A2, CYP99A3, and OsMAS) or production of momilactones upon elicitor treatment
  • CYP99A2, OsCPS4~OsCyc1, OsTGAP1, a bZIP transcription factor, coordinately regulates the inductive production of diterpenoid phytoalexins in rice, The knock-out mutant for OsTGAP1 had almost no expression of the five clustered genes (OsCPS4, OsKSL4, CYP99A2, CYP99A3, and OsMAS) or production of momilactones upon elicitor treatment
  • OsCPS4~OsCyc1, OsDTS2~OsKSL4~OsKS4, OsTGAP1, a bZIP transcription factor, coordinately regulates the inductive production of diterpenoid phytoalexins in rice, The knock-out mutant for OsTGAP1 had almost no expression of the five clustered genes (OsCPS4, OsKSL4, CYP99A2, CYP99A3, and OsMAS) or production of momilactones upon elicitor treatment
  • OsCPS4~OsCyc1, OsTGAP1~OsbZIP37, OsTGAP1, a bZIP transcription factor, coordinately regulates the inductive production of diterpenoid phytoalexins in rice, The knock-out mutant for OsTGAP1 had almost no expression of the five clustered genes (OsCPS4, OsKSL4, CYP99A2, CYP99A3, and OsMAS) or production of momilactones upon elicitor treatment
  • MAS, OsCPS4~OsCyc1, OsTGAP1, a bZIP transcription factor, coordinately regulates the inductive production of diterpenoid phytoalexins in rice, The knock-out mutant for OsTGAP1 had almost no expression of the five clustered genes (OsCPS4, OsKSL4, CYP99A2, CYP99A3, and OsMAS) or production of momilactones upon elicitor treatment
  • OsCPS2~OsCyc2, OsCPS4~OsCyc1, gid1, a gibberellin-insensitive dwarf mutant, shows altered regulation of probenazole-inducible protein PBZ1 in response to cold stress and pathogen attack, Specifically, OsCPS1, which encodes an enzyme in the GA biosynthesis pathway, was down-regulated and OsCPS2 and OsCPS4, which encode enzymes in phytoalexin biosynthesis, were up-regulated in gid1
  • GID1~OsGID1, OsCPS4~OsCyc1, gid1, a gibberellin-insensitive dwarf mutant, shows altered regulation of probenazole-inducible protein PBZ1 in response to cold stress and pathogen attack, Specifically, OsCPS1, which encodes an enzyme in the GA biosynthesis pathway, was down-regulated and OsCPS2 and OsCPS4, which encode enzymes in phytoalexin biosynthesis, were up-regulated in gid1
  • OsCPS~OsCPS1, OsCPS4~OsCyc1, gid1, a gibberellin-insensitive dwarf mutant, shows altered regulation of probenazole-inducible protein PBZ1 in response to cold stress and pathogen attack, Specifically, OsCPS1, which encodes an enzyme in the GA biosynthesis pathway, was down-regulated and OsCPS2 and OsCPS4, which encode enzymes in phytoalexin biosynthesis, were up-regulated in gid1
  • OsCPS2~OsCyc2, OsCPS4~OsCyc1, Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis, To clarify the biological functions of the ent- and syn-CDP synthases involved in the biosynthesis of phytoalexins and/or GAs, we isolated two cDNAs, OsCyc1 and OsCyc2, encoding putative diterpene cyclases from ultraviolet (UV)-irradiated rice leaves (cv
  • OsCPS2~OsCyc2, OsCPS4~OsCyc1, Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis, Using a bacterial expression system, we demonstrated that OsCyc1 encodes syn-CDP synthase and that OsCyc2 and OsCPS1 encode ent-CDP synthase
  • OsCPS2~OsCyc2, OsCPS4~OsCyc1, Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis, The level of expression of the OsCyc1 and OsCyc2 transcripts in rice leaves increased drastically in response to UV treatment, whereas expression of the OsCPS1 transcript was not induced by UV light
  • OsCPS2~OsCyc2, OsCPS4~OsCyc1, Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis, These results suggest that OsCyc1, OsCyc2 and OsCPS1 are responsible for the biosynthesis of momilactones A and B and oryzalexin S, oryzalexins A-F and phytocassanes A-E, and GAs, respectively
  • OsCPS~OsCPS1, OsCPS4~OsCyc1, Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis, Using a bacterial expression system, we demonstrated that OsCyc1 encodes syn-CDP synthase and that OsCyc2 and OsCPS1 encode ent-CDP synthase
  • OsCPS~OsCPS1, OsCPS4~OsCyc1, Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis, The level of expression of the OsCyc1 and OsCyc2 transcripts in rice leaves increased drastically in response to UV treatment, whereas expression of the OsCPS1 transcript was not induced by UV light
  • OsCPS~OsCPS1, OsCPS4~OsCyc1, Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis, These results suggest that OsCyc1, OsCyc2 and OsCPS1 are responsible for the biosynthesis of momilactones A and B and oryzalexin S, oryzalexins A-F and phytocassanes A-E, and GAs, respectively
  • OsCPS4~OsCyc1, OsDTS2~OsKSL4~OsKS4, Genetic evidence for natural product-mediated plant-plant allelopathy in rice Oryza sativa, Here, we apply reverse genetics, using knock-outs of the relevant diterpene synthases (copalyl diphosphate synthase 4 (OsCPS4) and kaurene synthase-like 4 (OsKSL4)), to demonstrate that rice momilactones are involved in allelopathy, including suppressing growth of the widespread rice paddy weed, barnyard grass (Echinochloa crus-galli)

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