- Information
- Symbol: OsGEN-L,OsGEN1
- MSU: LOC_Os09g35000
- RAPdb: Os09g0521900
- PSP score
- LOC_Os09g35000.1: 0.6607
- PLAAC score
- LOC_Os09g35000.1: 0
- pLDDT score
- 75.78
- Protein Structure from AlphaFold and UniProt
- Publication
- [The OsGEN-L protein from Oryza sativa possesses Holliday junction resolvase activity as well as 5 ‘-flap endonuclease activity](http://www.ncbi.nlm.nih.gov/pubmed?term=The OsGEN-L protein from Oryza sativa possesses Holliday junction resolvase activity as well as 5 ‘-flap endonuclease activity%5BTitle%5D), 2012, J Biochem.
- RNAi-mediated silencing of OsGEN-L OsGEN-like, a new member of the RAD2/XPG nuclease family, causes male sterility by defect of microspore development in rice, 2005, Plant Cell Physiol.
- Resolvase OsGEN1 mediates DNA repair by homologous recombination., 2017, Plant Physiol.
- Genbank accession number
- Key message
- OsGEN-L-RNAi plants lacked mature pollen, resulting from a defect in early microspore development
- Our results suggest that OsGEN-L plays an essential role in DNA metabolism required for early microspore development in rice
- RNAi-mediated silencing of OsGEN-L (OsGEN-like), a new member of the RAD2/XPG nuclease family, causes male sterility by defect of microspore development in rice
- Most of the OsGEN-L-RNAi plants displayed low fertility, and some of them were male-sterile
- OsGEN-L has a 5’-flap endonuclease activity and plays an essential role in rice microspore development
- A OsGEN-L-green fluorescent protein (GFP) fusion protein was localized in the nucleus, and the OsGEN-L promoter was specifically active in the anthers
- In contrast, depletion of OsSEND1 had no effects on plant development and did not enhance osgen1 defects
- We first investigated the role of OsGEN1 during meiosis and found a reduction in chiasma frequency by ~6% in osgen1 mutants, compared to wild type, suggesting a possible involvement of OsGEN1 in the formation of crossovers
- Post-meiosis, OsGEN1 foci were detected in wild-type microspore nuclei, but not in the osgen1 mutant concomitant with an increase in double strand breaks
- Connection
- OsGEN-L~OsGEN1, OsSEND-1~OsSEND1, Resolvase OsGEN1 Mediates DNA Repair by Homologous Recombination., In this study, we characterized the loss of function mutants of OsGEN1 and OsSEND1, a pair of paralogs of Yen1/GEN1 in rice (Oryza sativa)
- OsGEN-L~OsGEN1, OsSEND-1~OsSEND1, Resolvase OsGEN1 Mediates DNA Repair by Homologous Recombination., In contrast, depletion of OsSEND1 had no effects on plant development and did not enhance osgen1 defects
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