OsIRT1

2022-11-15 | Categories genes  | Tags ethylene  transporter  root  iron  transcription factor  homeostasis  cadmium  zinc  mitochondria  grain  growth 

• Information
  • Symbol: OsIRT1
  • MSU: LOC_Os03g46470
  • RAPdb: Os03g0667500
• PSP score
  • LOC_Os03g46470.1: 0.1499
• PLAAC score
  • LOC_Os03g46470.1: 0
• pLDDT score
  • 77.35
• Protein Structure from AlphaFold and UniProt
  • 
• Publication
  • Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, 2011, J Exp Bot.
  • Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+, 2006, Plant J.
  • OsZIP4, a novel zinc-regulated zinc transporter in rice, 2005, J Exp Bot.
  • Cloning an iron-regulated metal transporter from rice, 2002, J Exp Bot.
  • Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants, 2006, J Exp Bot.
  • A receptor-like protein RMC is involved in regulation of iron acquisition in rice, 2013, J Exp Bot.
  • Over-expression of OsIRT1 leads to increased iron and zinc accumulations in rice, 2009, Plant Cell Environ.
  • The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes, 2009, Plant J.
  • OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice Oryza sativa, 2012, New Phytol.
  • Iron deficiency enhances cadmium uptake and translocation mediated by the Fe2+transporters OsIRT1 and OsIRT2 in rice, 2006, Soil Science and Plant Nutrition.
  • Mutational reconstructed ferric chelate reductase confers enhanced tolerance in rice to iron deficiency in calcareous soil, 2007, Proc Natl Acad Sci U S A.
• Genbank accession number
  • AB070226
• Key message
  • RNA interference of OsIRO2 in transgenic rice showed that ethylene acted via this transcription factor to induce the expression of OsNAS1, OsNAS2, OsYSL15, and OsIRT1
  • This suggests that OsIRT1 is a functional metal transporter for iron, and is actively engaged in Fe uptake from soils, especially under limiting conditions
  • The knockout of OsARF12 also altered the abundance of mitochondrial iron-regulated (OsMIR), iron (Fe)-regulated transporter1 (OsIRT1) and short postembryonic root1 (OsSPR1) in roots of rice, and resulted in lower Fe content
  • In spite of being a Strategy II plant, however, rice (Oryza sativa) contains the previously identified Fe2+ transporter OsIRT1
  • Real-time PCR analysis revealed that OsIRT1 and OsIRT2 are expressed predominantly in roots, and these transporters are induced by low-Fe conditions
  • Over-expression of OsIRT1 leads to increased iron and zinc accumulations in rice
  • Northern blot analysis revealed that OsIRT1 mRNA was predominantly expressed in roots and was induced by Fe and Cu‐deficiency
  • Yeast mutants expressing OsIRT1 and OsIRT2, which encode the rice Fe2+ transporter, became more sensitive to Cd, suggesting that Cd was absorbed by OsIRT1 and OsIRT2
  • Iron deficiency enhances cadmium uptake and translocation mediated by the Fe2+transporters OsIRT1 and OsIRT2 in rice
  • A yeast Fe3+ chelate-reductase gene refre1/372, selected for better performance at high pH, was fused to the promoter of the Fe-regulated transporter, OsIRT1, and introduced into rice plants
  • We generated transgenic rice plants over-expressing OsIRT1 to evaluate its functional roles in metal homeostasis
  • OsIRT1 promoter-GUS analysis revealed that OsIRT1 is expressed in the epidermis and exodermis of the elongating zone and in the inner layer of the cortex of the mature zone of Fe-deficient roots
  • Four distinct genes, OsZIP4, OsZIP5, OsZIP6, and OsZIP7 that exhibit sequence similarity to the rice ferrous ion transporter, OsIRT1, were isolated
  • This demonstrates that OsIRT1 can be used for enhancing micronutrient levels in rice grains
  • When expressed in yeast cells, OsIRT1 cDNA reversed the growth defects of the yeast iron‐uptake mutant
• Connection
  • OsIRT1, OsNAS2, Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, RNA interference of OsIRO2 in transgenic rice showed that ethylene acted via this transcription factor to induce the expression of OsNAS1, OsNAS2, OsYSL15, and OsIRT1
  • OsIRT1, OsNAS2, Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, Gene expression analysis of rice iron-regulated bHLH transcription factor OsIRO2, nicotianamine synthases 1 and 2 (NAS1 and NAS2), yellow-stripe like transporter 15 (YSL15) and iron-regulated transporter (IRT1) indicated that ethylene caused an increase in transcript abundance of both Fe (II) and Fe (III)-phytosiderophore uptake systems
  • OsIRT1, OsNAS1, Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, RNA interference of OsIRO2 in transgenic rice showed that ethylene acted via this transcription factor to induce the expression of OsNAS1, OsNAS2, OsYSL15, and OsIRT1
  • OsIRT1, OsNAS1, Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, Gene expression analysis of rice iron-regulated bHLH transcription factor OsIRO2, nicotianamine synthases 1 and 2 (NAS1 and NAS2), yellow-stripe like transporter 15 (YSL15) and iron-regulated transporter (IRT1) indicated that ethylene caused an increase in transcript abundance of both Fe (II) and Fe (III)-phytosiderophore uptake systems
  • OsIRO2, OsIRT1, Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, Gene expression analysis of rice iron-regulated bHLH transcription factor OsIRO2, nicotianamine synthases 1 and 2 (NAS1 and NAS2), yellow-stripe like transporter 15 (YSL15) and iron-regulated transporter (IRT1) indicated that ethylene caused an increase in transcript abundance of both Fe (II) and Fe (III)-phytosiderophore uptake systems
  • OsIRO2, OsIRT1, Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, RNA interference of OsIRO2 in transgenic rice showed that ethylene acted via this transcription factor to induce the expression of OsNAS1, OsNAS2, OsYSL15, and OsIRT1
  • OsIRT1, OsYSL15, Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, RNA interference of OsIRO2 in transgenic rice showed that ethylene acted via this transcription factor to induce the expression of OsNAS1, OsNAS2, OsYSL15, and OsIRT1
  • OsIRT1, OsYSL15, Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, Gene expression analysis of rice iron-regulated bHLH transcription factor OsIRO2, nicotianamine synthases 1 and 2 (NAS1 and NAS2), yellow-stripe like transporter 15 (YSL15) and iron-regulated transporter (IRT1) indicated that ethylene caused an increase in transcript abundance of both Fe (II) and Fe (III)-phytosiderophore uptake systems
  • OsIRT1, OsIRT2, Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+, In this study, we isolated the OsIRT2 gene from rice, which is highly homologous to OsIRT1
  • OsIRT1, OsIRT2, Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+, Real-time PCR analysis revealed that OsIRT1 and OsIRT2 are expressed predominantly in roots, and these transporters are induced by low-Fe conditions
  • OsIRT1, OsIRT2, Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+, OsIRT1- and OsIRT2-green fluorescent protein fusion proteins localized to the plasma membrane when transiently expressed in onion (Allium cepa L
  • OsIRT1, OsZIP5, OsZIP4, a novel zinc-regulated zinc transporter in rice, Four distinct genes, OsZIP4, OsZIP5, OsZIP6, and OsZIP7 that exhibit sequence similarity to the rice ferrous ion transporter, OsIRT1, were isolated
  • OsIRT1, OsZIP4, OsZIP4, a novel zinc-regulated zinc transporter in rice, Four distinct genes, OsZIP4, OsZIP5, OsZIP6, and OsZIP7 that exhibit sequence similarity to the rice ferrous ion transporter, OsIRT1, were isolated
  • OsAPT1~APRT, OsIRT1, Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants, Sequences similar to the OsIRO2-binding sequence were found upstream of several genes that are involved in Fe acquisition, such as OsNAS1, OsNAS3, OsIRT1, OsFDH, OsAPT1, and IDS3
  • FDH, OsIRT1, Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants, Sequences similar to the OsIRO2-binding sequence were found upstream of several genes that are involved in Fe acquisition, such as OsNAS1, OsNAS3, OsIRT1, OsFDH, OsAPT1, and IDS3
  • OsIRO2, OsIRT1, Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants, Sequences similar to the OsIRO2-binding sequence were found upstream of several genes that are involved in Fe acquisition, such as OsNAS1, OsNAS3, OsIRT1, OsFDH, OsAPT1, and IDS3
  • OsIRT1, OsNAS3, Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants, Sequences similar to the OsIRO2-binding sequence were found upstream of several genes that are involved in Fe acquisition, such as OsNAS1, OsNAS3, OsIRT1, OsFDH, OsAPT1, and IDS3
  • OsIRT1, OsNAS1, Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants, Sequences similar to the OsIRO2-binding sequence were found upstream of several genes that are involved in Fe acquisition, such as OsNAS1, OsNAS3, OsIRT1, OsFDH, OsAPT1, and IDS3
  • OsIRT1, OsNAS1, A receptor-like protein RMC is involved in regulation of iron acquisition in rice, Several Fe deficiency-responsive genes including OsDMAS1, OsNAS1, OsNAS2, OsNAAT1, OsIRT1, OsYSL15, and OsIRO2 were up- and downregulated in OsRMC-overexpressing and RNAi plants compared with WT rice plants
  • OsIRT1, OsNAS2, A receptor-like protein RMC is involved in regulation of iron acquisition in rice, Several Fe deficiency-responsive genes including OsDMAS1, OsNAS1, OsNAS2, OsNAAT1, OsIRT1, OsYSL15, and OsIRO2 were up- and downregulated in OsRMC-overexpressing and RNAi plants compared with WT rice plants
  • OsIRO2, OsIRT1, A receptor-like protein RMC is involved in regulation of iron acquisition in rice, Several Fe deficiency-responsive genes including OsDMAS1, OsNAS1, OsNAS2, OsNAAT1, OsIRT1, OsYSL15, and OsIRO2 were up- and downregulated in OsRMC-overexpressing and RNAi plants compared with WT rice plants
  • OsIRT1, OsRMC~OsRLK, A receptor-like protein RMC is involved in regulation of iron acquisition in rice, Several Fe deficiency-responsive genes including OsDMAS1, OsNAS1, OsNAS2, OsNAAT1, OsIRT1, OsYSL15, and OsIRO2 were up- and downregulated in OsRMC-overexpressing and RNAi plants compared with WT rice plants
  • OsIRT1, OsYSL15, A receptor-like protein RMC is involved in regulation of iron acquisition in rice, Several Fe deficiency-responsive genes including OsDMAS1, OsNAS1, OsNAS2, OsNAAT1, OsIRT1, OsYSL15, and OsIRO2 were up- and downregulated in OsRMC-overexpressing and RNAi plants compared with WT rice plants
  • OsIRT1, OsNAAT1, A receptor-like protein RMC is involved in regulation of iron acquisition in rice, Several Fe deficiency-responsive genes including OsDMAS1, OsNAS1, OsNAS2, OsNAAT1, OsIRT1, OsYSL15, and OsIRO2 were up- and downregulated in OsRMC-overexpressing and RNAi plants compared with WT rice plants
  • OsIRO2, OsIRT1, The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes, Time-course expression analyses using quantitative reverse-transcriptase PCR revealed that the IDEF1 expression level was positively correlated with the level of induction of the Fe utilization-related genes OsIRO2, OsYSL15, OsIRT1, OsYSL2, OsNAS1, OsNAS2, OsNAS3 and OsDMAS1, just after the onset of Fe starvation
  • OsIRT1, OsNAS2, The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes, Time-course expression analyses using quantitative reverse-transcriptase PCR revealed that the IDEF1 expression level was positively correlated with the level of induction of the Fe utilization-related genes OsIRO2, OsYSL15, OsIRT1, OsYSL2, OsNAS1, OsNAS2, OsNAS3 and OsDMAS1, just after the onset of Fe starvation
  • IDEF1, OsIRT1, The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes, Time-course expression analyses using quantitative reverse-transcriptase PCR revealed that the IDEF1 expression level was positively correlated with the level of induction of the Fe utilization-related genes OsIRO2, OsYSL15, OsIRT1, OsYSL2, OsNAS1, OsNAS2, OsNAS3 and OsDMAS1, just after the onset of Fe starvation
  • OsIRT1, OsNAS3, The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes, Time-course expression analyses using quantitative reverse-transcriptase PCR revealed that the IDEF1 expression level was positively correlated with the level of induction of the Fe utilization-related genes OsIRO2, OsYSL15, OsIRT1, OsYSL2, OsNAS1, OsNAS2, OsNAS3 and OsDMAS1, just after the onset of Fe starvation
  • OsIRT1, OsNAS1, The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes, Time-course expression analyses using quantitative reverse-transcriptase PCR revealed that the IDEF1 expression level was positively correlated with the level of induction of the Fe utilization-related genes OsIRO2, OsYSL15, OsIRT1, OsYSL2, OsNAS1, OsNAS2, OsNAS3 and OsDMAS1, just after the onset of Fe starvation
  • OsIRT1, OsYSL2, The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes, Time-course expression analyses using quantitative reverse-transcriptase PCR revealed that the IDEF1 expression level was positively correlated with the level of induction of the Fe utilization-related genes OsIRO2, OsYSL15, OsIRT1, OsYSL2, OsNAS1, OsNAS2, OsNAS3 and OsDMAS1, just after the onset of Fe starvation
  • OsIRT1, OsYSL15, The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes, Time-course expression analyses using quantitative reverse-transcriptase PCR revealed that the IDEF1 expression level was positively correlated with the level of induction of the Fe utilization-related genes OsIRO2, OsYSL15, OsIRT1, OsYSL2, OsNAS1, OsNAS2, OsNAS3 and OsDMAS1, just after the onset of Fe starvation
  • OsARF12, OsIRT1, OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice Oryza sativa, The knockout of OsARF12 also altered the abundance of mitochondrial iron-regulated (OsMIR), iron (Fe)-regulated transporter1 (OsIRT1) and short postembryonic root1 (OsSPR1) in roots of rice, and resulted in lower Fe content
  • OsIRT1, Osspr1, OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice Oryza sativa, The knockout of OsARF12 also altered the abundance of mitochondrial iron-regulated (OsMIR), iron (Fe)-regulated transporter1 (OsIRT1) and short postembryonic root1 (OsSPR1) in roots of rice, and resulted in lower Fe content
  • OsIRT1, OsIRT2, Iron deficiency enhances cadmium uptake and translocation mediated by the Fe2+transporters OsIRT1 and OsIRT2 in rice, Yeast mutants expressing OsIRT1 and OsIRT2, which encode the rice Fe2+ transporter, became more sensitive to Cd, suggesting that Cd was absorbed by OsIRT1 and OsIRT2
  • OsIRT1, OsIRT2, Iron deficiency enhances cadmium uptake and translocation mediated by the Fe2+transporters OsIRT1 and OsIRT2 in rice, Iron deficiency enhances cadmium uptake and translocation mediated by the Fe2+transporters OsIRT1 and OsIRT2 in rice
  • OsIRT1, OsRab6a, A Small GTPase, OsRab6a, Is Involved in the Regulation of Iron Homeostasis in Rice, Exposure to Fe-deficient medium led to up-regulation of OsIRO2, OsIRT1, OsNAS1 and OsNAS2 in both wild-type and the transgenic rice plants, with the magnitude of up-regulation positively correlated with the expression levels of OsRab6a

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