- Information
- Symbol: OsKOS1,OsKOL4,CYP701A8
- MSU: LOC_Os06g37300
- RAPdb: Os06g0569500
- PSP score
- LOC_Os06g37300.2: 0.0147
- LOC_Os06g37300.1: 0.0362
- PLAAC score
- LOC_Os06g37300.2: 0
- LOC_Os06g37300.1: 0
- pLDDT score
- NA
- Publication
- A rice semi-dwarf gene, Tan-Ginbozu D35, encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase, 2004, Plant Mol Biol.
- The rice dwarf virus P2 protein interacts with ent-kaurene oxidases in vivo, leading to reduced biosynthesis of gibberellins and rice dwarf symptoms, 2005, Plant Physiol.
- CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism, 2012, Plant Physiol.
- Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis., 2015, Appl Microbiol Biotechnol.
- Genbank accession number
- Key message
- Introduction of OsKOL4 into d35Tan-Ginbozu did not rescue its dwarf phenotype, indicating that OsKOL4 is not involved in GA biosynthesis
- In particular, under conditions where OsKO2 catalyzes the expected conversion of ent-kaurene to ent-kaurenoic acid required for GA biosynthesis, OsKOL4 instead efficiently reacts with ent-sandaracopimaradiene and ent-cassadiene to produce the corresponding C3alpha-hydroxylated diterpenoids
- Thus, it appears that OsKOL4 plays a role in the more specialized diterpenoid metabolism of rice, and our results provide evidence for divergence of a KO/CYP701 family member from GA biosynthesis
- Connection
- OsKOS1~OsKOL4~CYP701A8, OsKOS2~OsKOL5, A rice semi-dwarf gene, Tan-Ginbozu D35, encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase, Expression analysis revealed that OsKOL2 and OsKOL4 were actively transcribed in various organs, while OsKOL1 and OsKOL5 were expressed only at low levels; OsKOL3 may be a pseudogene
- OsKOS1~OsKOL4~CYP701A8, OsKOS2~OsKOL5, A rice semi-dwarf gene, Tan-Ginbozu D35, encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase, OsKOL4 and OsKOL5 are likely to take part in phytoalexin biosynthesis, because their expression was promoted by UV irradiation and/or elicitor treatment
- D35~OsKOS3~OsKO2, OsKOS1~OsKOL4~CYP701A8, CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism, In particular, under conditions where OsKO2 catalyzes the expected conversion of ent-kaurene to ent-kaurenoic acid required for GA biosynthesis, OsKOL4 instead efficiently reacts with ent-sandaracopimaradiene and ent-cassadiene to produce the corresponding C3alpha-hydroxylated diterpenoids
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