- Information
- Symbol: OsPUL
- MSU: None
- RAPdb: None
- PSP score
- Not available
- PLAAC score
- Not available
- pLDDT score
- NA
- Publication
- Characterization of expression of the OsPUL gene encoding a pullulanase-type debranching enzyme during seed development and germination in rice, 2009, Plant Physiol Biochem.
- Structural and enzymatic characterization of the isoamylase1 homo-oligomer and the isoamylase1-isoamylase2 hetero-oligomer from rice endosperm, 2006, Planta.
- Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis, 2011, Plant Cell Physiol.
- Genbank accession number
- Key message
- The expression of the OsPUL gene was maintained at a significant level during the entire grain filling period and in the late stage of seed germination, which coincided with its involvement in starch anabolism and catabolism
- The OsPUL gene maintained a relatively high expression level during the entire period of seed development, and peaked in the middle and late stages
- Characterization of expression of the OsPUL gene encoding a pullulanase-type debranching enzyme during seed development and germination in rice
- In addition, the 1177-bp OsPUL promoter sequence was cloned, and some endosperm-specific motifs such as the GCN4 and AACA motifs were observed to exist in this region
- The data from both histochemical and fluorometric analyses showed that the OsPUL promoter was capable of driving the target gene to have a high level of endosperm-specific expression
- Furthermore, the seed germination experiment showed that the OsPUL promoter actively functions in the late stage of seed germination
- In the present study, we performed a quantitative real-time PCR (Q-PCR) analysis of the expression pattern of the OsPUL gene encoding a pullulanase-type DBE in different tissues as well as in seeds at different developmental stages
- Connection
- FLO2, OsPUL, Three novel alleles of FLOURY ENDOSPERM2 FLO2 confer dull grains with low amylose content in rice., The allelic diversity of flo2 perturbed the expression of starch synthesis-related genes including OsAGPL2, OsAGPS2b, OsGBSSI, OsBEI, OsBEIIb, OsISA1, and OsPUL
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