OsSPX-MFS3,OsPSS3

2022-11-15 | Categories genes  | Tags shoot  pi  transporter  plasma membrane  Pi 

• Information
  • Symbol: OsSPX-MFS3,OsPSS3
  • MSU: LOC_Os06g03860
  • RAPdb: Os06g0129400
• PSP score
  • LOC_Os06g03860.3: 0.0469
  • LOC_Os06g03860.4: 0.0233
  • LOC_Os06g03860.2: 0.0092
  • LOC_Os06g03860.1: 0.0705
• PLAAC score
  • LOC_Os06g03860.3: 0
  • LOC_Os06g03860.4: 0
  • LOC_Os06g03860.2: 0
  • LOC_Os06g03860.1: 0
• pLDDT score
  • 78.39
• Protein Structure from AlphaFold and UniProt
  • 
• Publication
  • Functional characterization of the rice SPX-MFS family reveals a key role of OsSPX-MFS1 in controlling phosphate homeostasis in leaves, 2012, New Phytol.
  • OsSPX-MFS3, a vacuolar phosphate efflux transporter, is involved in maintaining Pi homeostasis in rice., 2015, Plant Physiol.
• Genbank accession number
  • AK243620
• Key message
  • • Transcript analyses revealed that members of the SPX-MFS family were mainly expressed in the shoots, with OsSPX-MFS1 and OsSPX-MFS3 being suppressed by Pi deficiency, while OsSPX-MFS2 was induced
  • We conclude that OsSPX-MFS3 is a low affinity Pi transporter that mediates Pi efflux from the vacuole into cytosol and is coupled to proton movement
  • In contrast to tonoplast localization in plants cells, OsSPX-MFS3 was localised to the plasma membrane when expressed in both yeast and oocytes
  • At high external Pi concentration, OsSPX-MFS3 could partially complement the yeast (Saccharomyces cerevisiae) mutant strain EY917 under pH 5
  • In oocytes, OsSPX-MFS3 was shown to facilitate Pi influx or efflux depending on the external pH and Pi concentrations
  • Overexpression of OsSPX-MFS3 results in decreased Pi concentration in the vacuole of rice (Oryza sativa) tissues
• Connection
  • OsSPX-MFS1~OsPSS1, OsSPX-MFS3~OsPSS3, Functional characterization of the rice SPX-MFS family reveals a key role of OsSPX-MFS1 in controlling phosphate homeostasis in leaves, * Transcript analyses revealed that members of the SPX-MFS family were mainly expressed in the shoots, with OsSPX-MFS1 and OsSPX-MFS3 being suppressed by Pi deficiency, while OsSPX-MFS2 was induced
  • OsSPX-MFS1~OsPSS1, OsSPX-MFS3~OsPSS3, OsSPX-MFS3, a vacuolar phosphate efflux transporter, is involved in maintaining Pi homeostasis in rice., OsSPX-MFS1, OsSPX-MFS2 and OsSPX-MFS3 all localized on the tonoplast of rice protoplasts, even in the absence of the SPX domain
  • OsSPX-MFS2, OsSPX-MFS3~OsPSS3, OsSPX-MFS3, a vacuolar phosphate efflux transporter, is involved in maintaining Pi homeostasis in rice., OsSPX-MFS1, OsSPX-MFS2 and OsSPX-MFS3 all localized on the tonoplast of rice protoplasts, even in the absence of the SPX domain

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