OsSpo11-4,OsSPO11D

2022-11-15 | Categories genes  | Tags meiotic  pollen  anther  meiosis 

• Information
  • Symbol: OsSpo11-4,OsSPO11D
  • MSU: LOC_Os12g42760
  • RAPdb: Os12g0622500
• PSP score
  • LOC_Os12g42760.1: 0.0199
• PLAAC score
  • LOC_Os12g42760.1: 0
• pLDDT score
  • 76.98
• Protein Structure from AlphaFold and UniProt
  • 
• Publication
  • The double-stranded break-forming activity of plant SPO11s and a novel rice SPO11 revealed by a Drosophila bioassay, 2012, BMC Mol Biol.
  • OsSpo11-4, a rice homologue of the archaeal TopVIA protein, mediates double-strand DNA cleavage and interacts with OsTopVIB, 2011, PLoS One.
• Genbank accession number
  • AB219540
  • AK101363
  • AB219540
  • AK101363
• Key message
  • Quantitative analyses of the rice SPO11 transcripts revealed the specific increase in OsSPO11D mRNA in the anthers containing meiotic pollen mother cells
  • Furthermore, we identified a novel SPO11 homologue, OsSPO11D, with robust DSB activity and a possible meiotic function
  • We further analyzed the biological function of OsSpo11-4 by RNA interference and found that down-regulated expression of OsSpo11-4 led to defects in male meiosis, indicating OsSpo11-4 is required for meiosis
• Connection
  • OsSpo11-4~OsSPO11D, OsSpo11-5, OsSpo11-4, a rice homologue of the archaeal TopVIA protein, mediates double-strand DNA cleavage and interacts with OsTopVIB, Here, we further identified two novel Spo11/TopVIA homologues (named OsSpo11-4 and OsSpo11-5, respectively) that exist just in the monocot model plant Oryza sativa, indicating that at least five Spo11/TopVIA homologues are present in the rice genome
  • OsSpo11-4~OsSPO11D, OsSpo11-5, OsSpo11-4, a rice homologue of the archaeal TopVIA protein, mediates double-strand DNA cleavage and interacts with OsTopVIB, To reveal the biochemical function of the two novel Spo11/TopVIA homologues, we first examined the interactions among OsSpo11-1, OsSpo11-4, OsSpo11-5, and OsTopVIB by yeast two-hybrid assay

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