OsVIT2

2022-11-15 | Categories genes  | Tags transporter  manganese  mitochondria  seed  zinc  shoot  leaf  iron  growth  sheath 

• Information
  • Symbol: OsVIT2
  • MSU: LOC_Os09g23300
  • RAPdb: Os09g0396900
• PSP score
  • LOC_Os09g23300.1: 0.0128
• PLAAC score
  • LOC_Os09g23300.1: 0
• pLDDT score
  • 92.56
• Protein Structure from AlphaFold and UniProt
  • 
• Publication
  • The knockdown of OsVIT2 and MIT affects iron localization in rice seed, 2013, Rice (N Y).
  • Vacuolar membrane transporters OsVIT1 and OsVIT2 modulate iron translocation between flag leaves and seeds in rice, 2012, Plant J.
• Genbank accession number
  • AK071589
• Key message
  • Vacuolar membrane transporters OsVIT1 and OsVIT2 modulate iron translocation between flag leaves and seeds in rice
  • The accumulation of zinc, copper, and manganese also changed significantly in the shoots of osvit2 plants
  • Here, we discuss the characterization of a rice vacuolar Fe transporter 2 (OsVIT2) T-DNA insertion line (osvit2) and report that the knockdown of OsVIT2 and mitochondrial Fe transporter (MIT) expression affects seed Fe localization
  • The concentration of Fe increased in osvit2 polished seeds
  • Previously, we reported that the expression of OsVIT2 was higher in MIT knockdown (mit-2) plants, and in this study, the accumulation of Fe in mit-2 seeds decreased significantly
  • Moreover, changes in the expression of OsVIT2 and MIT affect the concentration and localization of metals in brown rice as well as in polished rice seeds
  • The knockdown of OsVIT2 and MIT affects iron localization in rice seed
  • Interestingly, functional disruption of OsVIT1 and OsVIT2 leads to increased Fe/Zn accumulation in rice seeds and a corresponding decrease in the source organ flag leaves, indicating an enhanced Fe/Zn translocation between source and sink organs, which might represent a novel strategy to biofortify Fe/Zn in staple foods
  • FINDINGS: osvit2 plants accumulated less Fe in their shoots when grown under normal or excess Fe conditions, while the accumulation of Fe was comparable to that in wild-type (WT) plants under Fe-deficient conditions
  • In rice, OsVIT1 and OsVIT2 are highly expressed in flag leaf blade and sheath, respectively, and in contrast to OsVIT1, OsVIT2 is highly responsive to Fe treatments
  • The growth of osvit2 plants was also slow compared to that of WT plants
• Connection
  • MIT, OsVIT2, The knockdown of OsVIT2 and MIT affects iron localization in rice seed, Here, we discuss the characterization of a rice vacuolar Fe transporter 2 (OsVIT2) T-DNA insertion line (osvit2) and report that the knockdown of OsVIT2 and mitochondrial Fe transporter (MIT) expression affects seed Fe localization
  • MIT, OsVIT2, The knockdown of OsVIT2 and MIT affects iron localization in rice seed, Previously, we reported that the expression of OsVIT2 was higher in MIT knockdown (mit-2) plants, and in this study, the accumulation of Fe in mit-2 seeds decreased significantly
  • MIT, OsVIT2, The knockdown of OsVIT2 and MIT affects iron localization in rice seed, Moreover, changes in the expression of OsVIT2 and MIT affect the concentration and localization of metals in brown rice as well as in polished rice seeds
  • MIT, OsVIT2, The knockdown of OsVIT2 and MIT affects iron localization in rice seed, The knockdown of OsVIT2 and MIT affects iron localization in rice seed
  • OsVIT1, OsVIT2, Vacuolar membrane transporters OsVIT1 and OsVIT2 modulate iron translocation between flag leaves and seeds in rice, In this study we report the isolation and functional characterization of two rice genes OsVIT1 and OsVIT2, orthologs of the Arabidopsis VIT1
  • OsVIT1, OsVIT2, Vacuolar membrane transporters OsVIT1 and OsVIT2 modulate iron translocation between flag leaves and seeds in rice, Transient expression of OsVIT1:EGFP and OsVIT2:EGFP protein fusions revealed that OsVIT1 and OsVIT2 are localized to the vacuolar membrane
  • OsVIT1, OsVIT2, Vacuolar membrane transporters OsVIT1 and OsVIT2 modulate iron translocation between flag leaves and seeds in rice, Ectopic expression of OsVIT1 and OsVIT2 partially rescued the Fe(2+) - and Zn(2+) -sensitive phenotypes in yeast mutant Deltaccc1 and Deltazrc1, and further increased vacuolar Fe(2+) , Zn(2+) and Mn(2+) accumulation
  • OsVIT1, OsVIT2, Vacuolar membrane transporters OsVIT1 and OsVIT2 modulate iron translocation between flag leaves and seeds in rice, These data together suggest that OsVIT1 and OsVIT2 function to transport Fe(2+) , Zn(2+) and Mn(2+) across the tonoplast into vacuoles in yeast
  • OsVIT1, OsVIT2, Vacuolar membrane transporters OsVIT1 and OsVIT2 modulate iron translocation between flag leaves and seeds in rice, In rice, OsVIT1 and OsVIT2 are highly expressed in flag leaf blade and sheath, respectively, and in contrast to OsVIT1, OsVIT2 is highly responsive to Fe treatments
  • OsVIT1, OsVIT2, Vacuolar membrane transporters OsVIT1 and OsVIT2 modulate iron translocation between flag leaves and seeds in rice, Interestingly, functional disruption of OsVIT1 and OsVIT2 leads to increased Fe/Zn accumulation in rice seeds and a corresponding decrease in the source organ flag leaves, indicating an enhanced Fe/Zn translocation between source and sink organs, which might represent a novel strategy to biofortify Fe/Zn in staple foods
  • OsVIT1, OsVIT2, Vacuolar membrane transporters OsVIT1 and OsVIT2 modulate iron translocation between flag leaves and seeds in rice, Vacuolar membrane transporters OsVIT1 and OsVIT2 modulate iron translocation between flag leaves and seeds in rice

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