OsYSL16

2022-11-15 | Categories genes  | Tags root  iron  seed  leaf  vascular bundle  transporter  spikelet  seedling  homeostasis  shoot 

• Information
  • Symbol: OsYSL16
  • MSU: LOC_Os04g45900
  • RAPdb: Os04g0542800
• PSP score
  • LOC_Os04g45900.1: 0.5605
• PLAAC score
  • LOC_Os04g45900.1: 0
• pLDDT score
  • 82.75
• Protein Structure from AlphaFold and UniProt
  • 
• Publication
  • Activation of rice Yellow Stripe1-Like 16 OsYSL16 enhances iron efficiency, 2012, Mol Cells.
  • OsYSL16 plays a role in the allocation of iron, 2012, Plant Mol Biol.
  • YSL16 is a phloem-localized transporter of the copper-nicotianamine complex that is responsible for copper distribution in rice, 2012, Plant Cell.
• Genbank accession number
  • AK070304
• Key message
  • Expression of the OsYSL16 promoter fused to the beta-glucuronidase gene showed that OsYSL16 is expressed in the root epidermis and vascular bundles of whole plants
  • Our results suggest that the function of OsYSL16 in Fe-homeostasis is to enable distribution of iron within a plant
  • Activation of rice Yellow Stripe1-Like 16 (OsYSL16) enhances iron efficiency
  • During germination, the rate of Fe-utilization from the seeds was higher in the OsYSL16 activation lines than in the WT seeds
  • Promoter fusions of OsYSL16 to beta-glucuronidase (GUS) showed that OsYSL16 was highly expressed in the vascular tissues of the root, leaf, and spikelet, and in leaf mesophyll cells
  • OsYSL16 has 85 % similarity to both OsYSL15 and the iron(II)-nicotianamine transporter OsYSL2
  • In the present study, we show that OsYSL16 functionally complemented a yeast mutant defective in iron uptake when grown on medium containing iron(III)-deoxymugineic acid, but not when grown on medium containing iron(II)-nicotianamine
  • OsYSL16-knockdown seedlings were smaller than wild-type seedlings when only iron(III)chloride was supplied as an iron source
  • The iron concentration in shoots of OsYSL16-knockdown plants was similar to that of the wild type; however, they showed more severe chlorosis than wild-type plants under iron-deficient conditions
  • Furthermore, OsYSL16-knockdown plants accumulated more iron in the vascular bundles of the leaves
  • Our results suggest that OsYSL16 plays a role in the allocation of iron(III)-deoxymugineic acid via the vascular bundles
  • OsYSL16 plays a role in the allocation of iron
  • The Fe concentration in shoots was also higher in the OsYSL16 activation lines than in the WT
• Connection

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