REP-1,OsEP3A

• Symbol: REP-1,OsEP3A
• MSU: LOC_Os01g67980
• RAPdb: Os01g0907600

• LOC_Os01g67980.1: 0.0136

• LOC_Os01g67980.1: 0

• NA

• Differential proteomic analysis of soluble extracellular proteins reveals the cysteine protease and cystatin involved in suspension-cultured cell proliferation in rice, 2009, Biochim Biophys Acta.
• Two cis-acting elements necessary and sufficient for gibberellin-upregulated proteinase expression in rice seeds, 2003, The Plant Journal.
• Multiple mode regulation of a cysteine proteinase gene expression in rice, 2000, Plant Physiol.

Genbank accession number

• In rice (Oryza sativa), REP-1 is a primary cysteine proteinase responsible for the digestion of seed storage proteins to provide nutrients to support the growth of young seedlings
• The expression of the gene for REP-1 (Rep1) is induced by gibberellins (GAs) and repressed by abscisic acid (ABA)
• An OsEP3A-specific DNA probe was used to study the effect of various factors on the expression of OsEP3A in germinating seeds and vegetative tissues of rice
• The expression of OsEP3A is hormonally regulated in germinating seeds, spatially and temporally regulated in vegetative tissues, and nitrogen-regulated in suspension-cultured cells
• Histochemical studies also indicate that the OsEP3A promoter is sufficient to confer the hormonal regulation of GUS expression in germinating seeds
• In germinating rice seeds, a cysteine proteinase (REP-1), synthesized in aleurone-layer cells, is a key enzyme in the degradation of the major storage protein, glutelin
• The OsEP3A promoter is sufficient to confer nitrogen regulation of GUS expression in suspension-cultured cells